https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18810 Sat 24 Mar 2018 07:51:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27726 Sat 24 Mar 2018 07:36:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29021 A/B human myometrial cell line, abundance of pSer345-PRA was induced by progesterone in a dose-(EC₅₀ ~1 nM) and time-dependent manner. Prevention of pSer345 (by site-directed mutagenesis) abolished the capacity for PR-A to inhibit anti-inflammatory actions of progesterone mediated by PR-B but had no effect on the transrepressive activity of PR-A at a canonical progesterone response element. Taken together, the data show that human parturition involves the phosphorylation of PR-A at serine-345 in myometrial cells and that this process is ligand dependent and induced by a proinflammatory stimulus. We also found that in myometrial cells, pSer345 activates the capacity for PR-A to inhibit antiinflammatory actions of progesterone mediated by PR-B. Phosphorylation of PR-A at serine-345 may be an important functional link between tissue-level inflammation and PR-A-mediated functional progesterone withdrawal to trigger parturition.]]> Sat 24 Mar 2018 07:31:08 AEDT ]]>